The corona virus has "accomplices", and mass spectrometry has a hand in chasing "crimes"!
At present, China's prevention and treatment of new coronavirus continues to improve, but the epidemic situation in other countries is spreading rapidly. According to the latest statistics released by Johns Hopkins University in the United States, the number of confirmed cases of new coronary pneumonia in the world has accumulated more than 1 million cases, and less than 20 countries have not yet had infections.
This sudden infectious disease has brought huge losses and threats to human beings, and medical workers have encountered many challenges in the process of diagnosis and treatment.
Corona virus has "accomplices"
In clinical practice, medical staff mainly use three methods to catch the new culprit SARS-CoV-2:
1 Real-time fluorescent RT-PCR to detect new coronavirus;
2 Sequencing with nucleic acid positive virus genes, highly homologous to known new coronaviruses;
3 New coronavirus-specific IgM antibodies and IgG positive or new coronavirus-specific IgG antibodies change from negative to positive or the recovery period is 4 times or more higher than the acute period.
However, many experts found in the follow-up treatment that the treatment of patients with new crowns must not only deal with SARS-CoV-2, but also other pathogenic microorganisms that cause co-infection, so that patients can fully recover.
The pathogenic microorganisms spread very quickly, which makes the patient's condition and treatment more difficult, and it becomes another major "accomplice" that hinders the patient's recovery. Among these "accomplices" that lead to co-infection, fungi are an important member.
On January 29, 2020, Lancet published a descriptive paper by Chinese scholars on the epidemiology and clinical characteristics of 99 patients with this new coronavirus pneumonia [1]. The article mentioned that 5 of 99 patients had fungal infections, of which 3 were diagnosed with Candida albicans infection, 1 was Candida glabrata infection, and the other was positive for Aspergillus flavus culture.
As early as the SARS outbreak in 2003, the sputum specimens of 33 SARS severe patients were detected by the 309 Hospital of the People's Liberation Army and found 6 cases of fungal co-infection and 3 cases of bacterial and fungal co-infection [2].
In the course of clinical treatment, cases of fungal co-infection have also been reported repeatedly, and their occurrence has greatly increased the mortality of patients.
Various data indicate that secondary fungal infections, whether occurring in SARS patients or in new crown patients, can seriously threaten the lives of patients. Therefore, the timely and accurate identification of pathogenic fungi and screening of effective therapeutic drugs is the urgent clinical need for laboratories.
Mass spectrometry "catch the murder"
For severe and critically ill patients with new coronavirus pneumonia, the focus of fungal infection monitoring is invasive pulmonary aspergillosis, pneumocystis pneumonia, cryptococcal pneumonia, central nervous system infection, pulmonary mucormycosis, candidemia, and other pathogenic fungi Infection [3];
Microbial mass spectrometry is a research hotspot in recent years, mainly used for rapid identification of bacteria, filamentous fungi, yeast, mycobacteria and so on. In addition to conventional bacterial identification, mass spectrometry detection of fungi has strong stability, good repeatability, simple and fast operation, low cost, many types of identification and accurate results. It is a fast and reliable method for clinical laboratory identification of fungi [4]. Shorten the reporting time of clinical microbiological detection of fungi.
At present, the pain points of mass detection of fungi are limited by the size of the filamentous fungal database, and the scope of application is limited.
In this field, Antu Biology has a database with independent intellectual property rights, of which there are more than 350 filamentous fungi. At the same time, it uses a multi-dimensional database construction model, taking into account different media, different cultivation time and other factors to ensure its identification in fungi Far ahead.
In this regard, Teacher Liang of Baise People's Hospital of Guangxi commented: "Antu mass spectrometer database can be updated in time for the identification of bacteria, and some newly discovered bacteria can also be identified. The particular satisfaction of this mass spectrometer is that it is difficult for bacteria such as Actinomycetes can correctly identify species, and the ability to identify fungi is the most prominent feature of this device. It has advantages over some foreign brands. It is more effective against Aspergillus, Fusarium, Candida, Cryptococcus, Mucor, Fungi such as Marneffe cyanobacteria can be identified without conventional treatment. Species can be identified according to routine operations. The scores are above 7 points. The mass spectrum is clear, the results are reliable, and the diagnostic compliance rate reaches 100%. "
Summary
Patients with new crowns secondary to fungal infections are prone to cause severe and critical illnesses. Severe new crown pneumonia is a key difficulty in the treatment of anti-epidemic work. Therefore, the sooner an “accomplice” is found in the diagnosis and treatment process, the more beneficial it is to the treatment of the patient.
Conventional fungal detection mainly relies on biochemical methods, and the limited number of bacterial identifications is slow and can not meet the clinical needs. Microbial mass spectrometry detection of fungi is a tool for chasing the "criminal" with fast, accurate and convenient operation, and is widely used in the treatment of infectious diseases Splendid.
This sudden infectious disease has brought huge losses and threats to human beings, and medical workers have encountered many challenges in the process of diagnosis and treatment.
Corona virus has "accomplices"
In clinical practice, medical staff mainly use three methods to catch the new culprit SARS-CoV-2:
1 Real-time fluorescent RT-PCR to detect new coronavirus;
2 Sequencing with nucleic acid positive virus genes, highly homologous to known new coronaviruses;
3 New coronavirus-specific IgM antibodies and IgG positive or new coronavirus-specific IgG antibodies change from negative to positive or the recovery period is 4 times or more higher than the acute period.
However, many experts found in the follow-up treatment that the treatment of patients with new crowns must not only deal with SARS-CoV-2, but also other pathogenic microorganisms that cause co-infection, so that patients can fully recover.
The pathogenic microorganisms spread very quickly, which makes the patient's condition and treatment more difficult, and it becomes another major "accomplice" that hinders the patient's recovery. Among these "accomplices" that lead to co-infection, fungi are an important member.
On January 29, 2020, Lancet published a descriptive paper by Chinese scholars on the epidemiology and clinical characteristics of 99 patients with this new coronavirus pneumonia [1]. The article mentioned that 5 of 99 patients had fungal infections, of which 3 were diagnosed with Candida albicans infection, 1 was Candida glabrata infection, and the other was positive for Aspergillus flavus culture.
As early as the SARS outbreak in 2003, the sputum specimens of 33 SARS severe patients were detected by the 309 Hospital of the People's Liberation Army and found 6 cases of fungal co-infection and 3 cases of bacterial and fungal co-infection [2].
In the course of clinical treatment, cases of fungal co-infection have also been reported repeatedly, and their occurrence has greatly increased the mortality of patients.
Various data indicate that secondary fungal infections, whether occurring in SARS patients or in new crown patients, can seriously threaten the lives of patients. Therefore, the timely and accurate identification of pathogenic fungi and screening of effective therapeutic drugs is the urgent clinical need for laboratories.
Mass spectrometry "catch the murder"
For severe and critically ill patients with new coronavirus pneumonia, the focus of fungal infection monitoring is invasive pulmonary aspergillosis, pneumocystis pneumonia, cryptococcal pneumonia, central nervous system infection, pulmonary mucormycosis, candidemia, and other pathogenic fungi Infection [3];
Microbial mass spectrometry is a research hotspot in recent years, mainly used for rapid identification of bacteria, filamentous fungi, yeast, mycobacteria and so on. In addition to conventional bacterial identification, mass spectrometry detection of fungi has strong stability, good repeatability, simple and fast operation, low cost, many types of identification and accurate results. It is a fast and reliable method for clinical laboratory identification of fungi [4]. Shorten the reporting time of clinical microbiological detection of fungi.
At present, the pain points of mass detection of fungi are limited by the size of the filamentous fungal database, and the scope of application is limited.
In this field, Antu Biology has a database with independent intellectual property rights, of which there are more than 350 filamentous fungi. At the same time, it uses a multi-dimensional database construction model, taking into account different media, different cultivation time and other factors to ensure its identification in fungi Far ahead.
In this regard, Teacher Liang of Baise People's Hospital of Guangxi commented: "Antu mass spectrometer database can be updated in time for the identification of bacteria, and some newly discovered bacteria can also be identified. The particular satisfaction of this mass spectrometer is that it is difficult for bacteria such as Actinomycetes can correctly identify species, and the ability to identify fungi is the most prominent feature of this device. It has advantages over some foreign brands. It is more effective against Aspergillus, Fusarium, Candida, Cryptococcus, Mucor, Fungi such as Marneffe cyanobacteria can be identified without conventional treatment. Species can be identified according to routine operations. The scores are above 7 points. The mass spectrum is clear, the results are reliable, and the diagnostic compliance rate reaches 100%. "
Summary
Patients with new crowns secondary to fungal infections are prone to cause severe and critical illnesses. Severe new crown pneumonia is a key difficulty in the treatment of anti-epidemic work. Therefore, the sooner an “accomplice” is found in the diagnosis and treatment process, the more beneficial it is to the treatment of the patient.
Conventional fungal detection mainly relies on biochemical methods, and the limited number of bacterial identifications is slow and can not meet the clinical needs. Microbial mass spectrometry detection of fungi is a tool for chasing the "criminal" with fast, accurate and convenient operation, and is widely used in the treatment of infectious diseases Splendid.
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